Full Product Name
MLKL antibody - N-terminal region
Product Gene Name
anti-MLKL antibody
[Similar Products]
Product Synonym Gene Name
FLJ34389[Similar Products]
Antibody/Peptide Pairs
MLKL peptide (MBS3236074) is used for blocking the activity of MLKL antibody (MBS3211122)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: DVWKELSLLL QVEQRMPVSP ISQGASWAQE DQQDADEDRR AFQMLRRDNE
3D Structure
ModBase 3D Structure for Q8NB16
Species Reactivity
Cow, Horse, Human, Rat
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Cow: 85%; Horse: 91%; Human: 100%; Rat: 90%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human MLKL
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-MLKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MLKL antibody
This is a rabbit polyclonal antibody against MLKL. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: The specific function of this protein remains unknown.
Product Categories/Family for anti-MLKL antibody
Polyclonal; Various;
Applications Tested/Suitable for anti-MLKL antibody
Western Blot (WB)
Western Blot (WB) of anti-MLKL antibody
Host: Rabbit
Target Name: MLKL
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml
Western Blot (WB) of anti-MLKL antibody
Host: Rabbit
Target Name: MLKL
Sample Tissue: Human Lung Tumor
Antibody Dilution: 1ug/ml
Western Blot (WB) of anti-MLKL antibody
Host: Rabbit
Target Name: MLKL
Sample Type: 293T Whole Cell lysates
Antibody Dilution: 1ug/ml
NCBI/Uniprot data below describe general gene information for MLKL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_689862
[Other Products]
NCBI GenBank Nucleotide #
NM_152649
[Other Products]
UniProt Primary Accession #
Q8NB16
[Other Products]
UniProt Related Accession #
Q8NB16[Other Products]
NCBI Official Full Name
mixed lineage kinase domain-like protein isoform 1
NCBI Official Synonym Full Names
mixed lineage kinase domain like pseudokinase
NCBI Official Symbol
MLKL??[Similar Products]
NCBI Official Synonym Symbols
hMLKL
??[Similar Products]
NCBI Protein Information
mixed lineage kinase domain-like protein
UniProt Protein Name
Mixed lineage kinase domain-like protein
Protein Family
Mixed lineage kinase domain-like protein
UniProt Gene Name
MLKL??[Similar Products]
UniProt Entry Name
MLKL_HUMAN
NCBI Summary for MLKL
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for MLKL
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; TKL-Unique family
Chromosomal Location of Human Ortholog: 16q23.1
Cellular Component: cytoplasm; plasma membrane
Molecular Function: protein binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity
Biological Process: protein amino acid phosphorylation
Research Articles on MLKL
1. Following activation, toggling within the MLKL pseudokinase domain promotes 4HB domain disengagement from the pseudokinase domain alphaC helix and pseudocatalytic loop, to enable formation of a necroptosis-inducing tetramer.
Precautions
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Disclaimer
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