Product Name
Collagen IV alpha4 (COL4A4), Polyclonal Antibody
Full Product Name
Collagen IV alpha4 Antibody
Product Synonym Names
Collagen alpha-4(IV) chain; COL4A4; CO4A4
Product Gene Name
anti-COL4A4 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P53420
Specificity
Collagen IV alpha4 Antibody detects endogenous levels of total Collagen IV alpha4 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen IV alpha4.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-COL4A4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COL4A4 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-COL4A4 antibody
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Immunofluorescence (IF) of anti-COL4A4 antibody
Immunofluorescence analysis of COS7 cells, using Collagen IV alpha4 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry (IHC) of anti-COL4A4 antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Collagen IV alpha4 Antibody. The picture on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for COL4A4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000083.3
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NCBI GenBank Nucleotide #
NM_000092.4
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UniProt Primary Accession #
P53420
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UniProt Secondary Accession #
Q53RW9; Q53S42; Q53WR1; A8MTZ1[Other Products]
UniProt Related Accession #
P53420[Other Products]
NCBI Official Full Name
collagen alpha-4(IV) chain
NCBI Official Synonym Full Names
collagen type IV alpha 4 chain
NCBI Official Symbol
COL4A4??[Similar Products]
NCBI Official Synonym Symbols
CA44
??[Similar Products]
NCBI Protein Information
collagen alpha-4(IV) chain
UniProt Protein Name
Collagen alpha-4(IV) chain
UniProt Gene Name
COL4A4??[Similar Products]
NCBI Summary for COL4A4
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]
UniProt Comments for COL4A4
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Research Articles on COL4A4
1. Alport syndrome is the result of mutations in any of three type IV collagen genes, COL4A3, COL4A4, or COL4A5. Because the three collagen chains form heterotrimers, there is an absence of all three proteins in the basement membranes where they are expressed. (Review)
Precautions
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Disclaimer
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