Product Name
Collagen IV alpha4 (COL4A4), Antibody
Full Product Name
Collagen IV alpha4 Antibody
Product Synonym Names
Collagen alpha-4(IV) chain; CO4A4
Product Gene Name
anti-COL4A4 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P53420
Specificity
Collagen IV alpha4 antibody detects endogenous levels of total Collagen IV alpha4 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human Collagen IV alpha4.
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-COL4A4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-COL4A4 antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-COL4A4 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-COL4A4 antibody
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Immunohistochemistry (IHC) of anti-COL4A4 antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Collagen IV alpha4 antibody.
Immunofluorescence (IF) of anti-COL4A4 antibody
Immunofluorescence analysis of COS-7 cells, using Collagen IV ?4 antibody.
NCBI/Uniprot data below describe general gene information for COL4A4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000083.3
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NCBI GenBank Nucleotide #
NM_000092.4
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UniProt Primary Accession #
P53420
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UniProt Secondary Accession #
Q53RW9; Q53S42; Q53WR1; A8MTZ1[Other Products]
UniProt Related Accession #
P53420[Other Products]
Molecular Weight
164,038 Da
NCBI Official Full Name
collagen alpha-4(IV) chain
NCBI Official Synonym Full Names
collagen type IV alpha 4 chain
NCBI Official Symbol
COL4A4??[Similar Products]
NCBI Official Synonym Symbols
CA44
??[Similar Products]
NCBI Protein Information
collagen alpha-4(IV) chain
UniProt Protein Name
Collagen alpha-4(IV) chain
UniProt Gene Name
COL4A4??[Similar Products]
NCBI Summary for COL4A4
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]
UniProt Comments for COL4A4
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Product References and Citations for anti-COL4A4 antibody
Leinonen A., J. Biol. Chem. 269:26172-26177(1994). Boye E., Am. J. Hum. Genet. 63:1329-1340(1998). Hillier L.W., Nature 434:724-731(2005).
Research Articles on COL4A4
1. Three collagen type IV alpha 4 chain (COL4A4) heterozygous mutations that lead to 3 different collagen type IV kidney disease phenotypes, manifesting as Thin basement membrane nephropathy (TBMN), autosomal dominant Alport syndrome (ADAS), and focal segmental glomerulosclerosis (FSGS).
Precautions
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Disclaimer
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