For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against ACTN2. It has been selected for its ability to recognize ACTN2 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-ACTN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Knock-Out Validation Antibody

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant protein.

DAB staining on fromalin fixed paraffin-embedded heart tissue)

Knockout Validation: Lane 1: Wild-type Hela cell lysate;;Lane 2: ACTN2 knockout Hela cell lysate;;Predicted MW: 104kDa ;Observed MW: 104kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse ACTN2 Antibody;Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)

103,834 Da

actinin alpha 2

alpha-actinin-2

Alpha-actinin-2

ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13 A1|13 4.54 cM

Cellular Component: cortical actin cytoskeleton; cytoplasm; filopodium; focal adhesion; plasma membrane; sarcomere; striated muscle thin filament; Z disc

Molecular Function: actin binding; actin filament binding; calcium ion binding; cytoskeletal protein binding; FATZ binding; identical protein binding; ligand-dependent nuclear receptor transcription coactivator activity; LIM domain binding; metal ion binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein binding, bridging; protein dimerization activity; protein domain specific binding; protein homodimerization activity; thyroid hormone receptor coactivator activity; titin binding

Biological Process: actin filament uncapping; cardiac muscle cell development; focal adhesion assembly; microspike assembly; muscle contraction; negative regulation of potassium ion transport; positive regulation of potassium ion transport; protein homotetramerization; regulation of membrane potential; sarcomere organization

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.