Human Retinoschisin (RS1) ELISA kit; RP1-245G19.1; RS; XLRS1; X-linked juvenile retinoschisis protein; retinoschisis (X-linked; juvenile) 1; retinoschisin 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human RS1. No significant cross-reactivity or interference between Human RS1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS926224 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the retinoschisin 1 (RS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RS1. The ELISA analytical biochemical technique of the MBS926224 kit is based on RS1 antibody-RS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

25,592 Da

retinoschisin 1

retinoschisin; X-linked juvenile retinoschisis protein

Retinoschisin

XLRS1??[Similar Products]

XLRS1_HUMAN

This gene encodes an extracellular protein that plays a crucial role in the cellular organization of the retina. The encoded protein is assembled and secreted from photoreceptors and bipolar cells as a homo-oligomeric protein complex. Mutations in this gene are responsible for X-linked retinoschisis, a common, early-onset macular degeneration in males that results in a splitting of the inner layers of the retina and severe loss in vision. [provided by RefSeq, Oct 2008]

RS1: a highly conserved extracellular protein essential for proper retinal structure. Must interact with L-type voltage-gated calcium channels (L-VGCCs) correctly so that the photoreceptor cells in the eye can function properly. Genetic mutations in RS1 cause X-linked retinoschisis (XLRS) and early onset of macular degeneration. Interacts with the L-VGCCa1D subunit, regulating its activity. The expression and secretion of retinoschisin are modulated by circadian rhythms, peaking at night and diminishing during the day.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp22.13

Cellular Component: extrinsic to plasma membrane; extracellular space

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylserine binding; phosphatidylinositol-5-phosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol 3-phosphate binding

Biological Process: visual perception; multicellular organismal development; adaptation of rhodopsin mediated signaling; cell adhesion

Disease: Retinoschisis 1, X-linked, Juvenile

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