Purified Rabbit Polyclonal Antibody (Pab)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ITGB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha- IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Cancer; Cardiovascular; Immunology; Signal Transduction; Stem Cells

Western Blot (WB)

WB ~~1:1000

Anti-CD61 Antibody at 1:1000 dilution + U-87 MG whole cell lysate

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 87 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

87058

integrin beta 3

integrin beta-3

Integrin beta-3

GP3A; GPIIIa??[Similar Products]

ITB3_HUMAN

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: filopodium membrane; platelet alpha granule membrane; focal adhesion; cell surface; integral to plasma membrane; microvillus membrane; melanosome; plasma membrane; nucleus; integrin complex; receptor complex

Molecular Function: identical protein binding; protein binding; enzyme binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; protein disulfide isomerase activity; platelet-derived growth factor receptor binding; cell adhesion molecule binding; receptor activity

Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; protein folding; wound healing; cell-matrix adhesion; smooth muscle cell migration; mesodermal cell differentiation; negative chemotaxis; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell growth; cell adhesion; integrin-mediated signaling pathway; platelet activation; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; tube development; leukocyte migration

Disease: Glanzmann Thrombasthenia; Myocardial Infarction, Susceptibility To; Bleeding Disorder, Platelet-type, 16

Fitzgerald L.A.,et al.J. Biol. Chem. 262:3936-3939(1987). Zimrin A.B.,et al.J. Clin. Invest. 81:1470-1475(1988). Frachet P.,et al.Mol. Biol. Rep. 14:27-33(1990). Kumar C.S.,et al.J. Biol. Chem. 272:16390-16397(1997). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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