For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RPS6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7242497 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 40S ribosomal protein S6 (RPS6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPS6. The ELISA analytical biochemical technique of the MBS7242497 kit is based on RPS6 antibody-RPS6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPS6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPS6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

28,681 Da

ribosomal protein S6

40S ribosomal protein S6; phosphoprotein NP33

40S ribosomal protein S6

OK/SW-cl.2??[Similar Products]

RS6_HUMAN

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: polysome; small ribosomal subunit; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; nucleolus; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; structural constituent of ribosome; protein kinase binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; mitosis; erythrocyte development; TOR signaling pathway; viral reproduction; translation; positive regulation of apoptosis; mitotic cell cycle checkpoint; T cell differentiation in the thymus; gastrulation; viral infectious cycle; translational termination; glucose homeostasis; oogenesis stage; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; T cell proliferation during immune response; insulin receptor signaling pathway; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; gene expression; viral transcription; activated T cell apoptosis; rRNA processing; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; placenta development

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