Product Name
Alpha-tubulin (TUBA1B), Monoclonal Antibody
Full Product Name
Anti-Alpha-tubulin (AcK40) Antibody
Product Synonym Names
Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Product Gene Name
anti-TUBA1B antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68363
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Alpha-tubulin (AcK40) protein.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-tubulin. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-TUBA1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBA1B antibody
Mouse monoclonal to Alpha-tubulin (AcK40)
Applications Tested/Suitable for anti-TUBA1B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TUBA1B antibody
Western Blot: (1/1000 - 1/2000); Immunohistochemistry: (1/100 - 1/200)
Western Blot (WB) of anti-TUBA1B antibody
Western blot analysis of Alpha-tubulin (AcK40) expression in HeLa (A) whole cell lysates.
Immunohistochemistry (IHC) of anti-TUBA1B antibody
Immunohistochemical analysis of Alpha-tubulin (AcK40) staining in human breast cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
NCBI/Uniprot data below describe general gene information for TUBA1B. It may not necessarily be applicable to this product.
NCBI Accession #
P68363.1
[Other Products]
UniProt Primary Accession #
P68363
[Other Products]
UniProt Secondary Accession #
P04687; P05209; Q27I68; Q8WU19[Other Products]
UniProt Related Accession #
P68363[Other Products]
Molecular Weight
37,218 Da
NCBI Official Full Name
Tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin alpha 1b
NCBI Official Symbol
TUBA1B??[Similar Products]
NCBI Official Synonym Symbols
K-ALPHA-1
??[Similar Products]
NCBI Protein Information
tubulin alpha-1B chain
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Synonym Protein Names
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Protein Family
Alpha-tubulin N-acetyltransferase
UniProt Gene Name
TUBA1B??[Similar Products]
UniProt Entry Name
TBA1B_HUMAN
UniProt Comments for TUBA1B
TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: cytoplasmic microtubule; microtubule; myelin sheath
Molecular Function: double-stranded RNA binding; GTP binding; GTPase activity; protein binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding
Biological Process: 'de novo' posttranslational protein folding; cell division; cellular protein metabolic process; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process; protein folding
Research Articles on TUBA1B
1. Results suggest that lithium chloride (LiCl) treatments activate alpha-tubulin N-acetyltransferase 1 (alphaTAT1) by the inhibition of glycogen synthase kinase 3 beta (GSK-3beta) and promote the alpha-tubulin acetylation, and then elongate the primary cilia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
