Product Name
Aromatase (CYP19A1), Polyclonal Antibody
Full Product Name
Aromatase Antibody
Product Synonym Names
25147; Cyp19a1; Aromatase; Cyp19; p450arom; ARO1; ARO 1; CPV; CYP19A1; MGC104309
Product Gene Name
anti-CYP19A1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P11511
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Horse
Form/Format
100 ug (0.2mg/ml) affinity purified rabbit anti-Aromatase polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid
Concentration
0.2 mg/ml (lot specific)
Immunogen
Synthetic peptide surrounding amino acid 385 of human Aromatase
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Other Notes
Small volumes of anti-CYP19A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CYP19A1 antibody
Background: Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, fertility, and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
Applications Tested/Suitable for anti-CYP19A1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CYP19A1 antibody
Western blotting (0.5-4 ug/ml), Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody should recognize aromatase in samples from human, mouse, rat, bovine, porcine, pig, sheep, rabbit, horse and chicken origins. A 55 kDa band can be detected, corresponding to the expected molecular weight of Aromatase. An additional 35 kDa band (believe to be the cleavage product of Aromatase) can also be detected in Jurkat cells.
NCBI/Uniprot data below describe general gene information for CYP19A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000094.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000103.3
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UniProt Primary Accession #
P11511
[Other Products]
UniProt Secondary Accession #
Q16731; Q3B764; Q58FA0; Q8IYJ7[Other Products]
UniProt Related Accession #
P11511[Other Products]
Molecular Weight
24,518 Da[Similar Products]
NCBI Official Full Name
aromatase
NCBI Official Synonym Full Names
cytochrome P450, family 19, subfamily A, polypeptide 1
NCBI Official Symbol
CYP19A1??[Similar Products]
NCBI Official Synonym Symbols
ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
??[Similar Products]
NCBI Protein Information
aromatase
UniProt Protein Name
Aromatase
UniProt Synonym Protein Names
CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase
UniProt Gene Name
CYP19A1??[Similar Products]
UniProt Synonym Gene Names
ARO1; CYAR; CYP19??[Similar Products]
UniProt Entry Name
CP19A_HUMAN
NCBI Summary for CYP19A1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Comments for CYP19A1
CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.
Protein type: Oxidoreductase; EC 1.14.14.14; Lipid Metabolism - androgen and estrogen
Chromosomal Location of Human Ortholog: 15q21.1
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum
Molecular Function: electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding
Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; estrogen biosynthetic process; sterol metabolic process; steroid biosynthetic process
Disease: Aromatase Deficiency; Aromatase Excess Syndrome
Research Articles on CYP19A1
1. Increased expression of aromatase P450 is associated with endometrial hyperplasia in polycystic ovary syndrome.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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