Full Product Name
TLR5 siRNA (Mouse)
Product Synonym Names
Toll-like receptor 5
Product Gene Name
TLR5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JLF7
Specificity
TLR5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TLR5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TLR5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TLR5 sirna
siRNA to inhibit TLR5 expression using RNA interference
Applications Tested/Suitable for TLR5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TLR5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058624.2
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NCBI GenBank Nucleotide #
NM_016928.3
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UniProt Primary Accession #
Q9JLF7
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UniProt Related Accession #
Q9JLF7[Other Products]
Molecular Weight
97,627 Da
NCBI Official Full Name
toll-like receptor 5
NCBI Official Synonym Full Names
toll-like receptor 5
NCBI Official Symbol
Tlr5??[Similar Products]
NCBI Protein Information
toll-like receptor 5
UniProt Protein Name
Toll-like receptor 5
Protein Family
Toll-like receptor
UniProt Gene Name
Tlr5??[Similar Products]
UniProt Entry Name
TLR5_MOUSE
UniProt Comments for TLR5
TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.
Protein type: Membrane protein, integral; Receptor, misc.
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding; interleukin-1 receptor binding; transmembrane receptor activity
Biological Process: positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; positive regulation of cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; immune system process; defense response to bacterium; innate immune response; signal transduction; inflammatory response; positive regulation of toll-like receptor signaling pathway
Research Articles on TLR5
1. TLR5 is important to limit myocardial damage, inflammation and functional compromise after myocardial ischemia-reperfusion.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
