Full Product Name
Rabbit Polyclonal (IgG) to Human TLR5
Product Synonym Names
Anti-TLR5 Antibody (aa300-350) IHC-plus; TLR5; TIL3; Toll-like receptor 5; SLEB1; Human TLR5
Product Gene Name
anti-TLR5 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60602
Species Reactivity
Human, Mouse, Rat
Specificity
KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 found between amino acids 300-350. It will cross-react with mouse and rat TLR5.
Purity/Purification
Protein G Purified
Form/Format
PBS containing 0.05% BSA and 0.05% sodium azide
Concentration
0.5 mg/ml (lot specific)
Immunogen Description
Synthetic peptide from human TLR5.
Immunogen Type
Synthetic peptide
Immunogen
TLR5 antibody was raised against synthetic peptide from human TLR5.
Antigen Modification
aa300-350
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Other Notes
Small volumes of anti-TLR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-TLR5 antibody
Family: Toll-like Receptor
Applications Tested/Suitable for anti-TLR5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-TLR5 antibody
Flo, IHC-P (10 ug/ml), WB (1 - 3 ug/ml)
Immunohistochemistry (IHC) of anti-TLR5 antibody
Anti-TLR5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Flow Cytometry (FC/FACS) of anti-TLR5 antibody
Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control; red represents TLR5 antibody. Goat anti-rabbit PE was used as the secondary antibody.

Western Blot (WB) of anti-TLR5 antibody
Western blot of TLR5 in (A) Ramos, (B) Raw, (C) mouse spleen, (D) mouse lung and (E) rat lung whole cell lysate using antibody at 2 ug/ml.

NCBI/Uniprot data below describe general gene information for TLR5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003259.2
[Other Products]
NCBI GenBank Nucleotide #
NM_003268.5
[Other Products]
UniProt Primary Accession #
O60602
[Other Products]
UniProt Secondary Accession #
O15456; Q32MI2; Q32MI3; B1AZ05; B3Y633; B9VJ63; D1CS80; D3DTB8[Other Products]
UniProt Related Accession #
O60602[Other Products]
Molecular Weight
97,834 Da
NCBI Official Full Name
toll-like receptor 5
NCBI Official Synonym Full Names
toll-like receptor 5
NCBI Official Symbol
TLR5??[Similar Products]
NCBI Official Synonym Symbols
SLE1; TIL3; SLEB1; MELIOS
??[Similar Products]
NCBI Protein Information
toll-like receptor 5; toll/interleukin-1 receptor-like protein 3; systemic lupus erythematosus susceptibility 1
UniProt Protein Name
Toll-like receptor 5
UniProt Synonym Protein Names
Toll/interleukin-1 receptor-like protein 3
Protein Family
Toll-like receptor
UniProt Gene Name
TLR5??[Similar Products]
UniProt Synonym Gene Names
TIL3??[Similar Products]
UniProt Entry Name
TLR5_HUMAN
NCBI Summary for TLR5
This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]
UniProt Comments for TLR5
TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: integral to membrane; plasma membrane
Molecular Function: transmembrane receptor activity; interleukin-1 receptor binding
Biological Process: positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; defense response to bacterium; male gonad development; toll-like receptor signaling pathway; innate immune response; inflammatory response; positive regulation of toll-like receptor signaling pathway; toll-like receptor 10 signaling pathway
Disease: Legionnaire Disease, Susceptibility To; Systemic Lupus Erythematosus, Susceptibility To, 1; Melioidosis, Susceptibility To
Research Articles on TLR5
1. results reveal broad expression patterns of TLR5 and TLR7 in the lung and that the expression is decreased in severe asthma.
Precautions
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