TLR5; TIL3; SLEB1; MELIOS; TIL3; Toll-like receptor 5; toll-like receptor 5

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

TLR5 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

TLR5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-TLR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TLR5 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-α and other cytokines. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires'disease and demonstrates the importance of TLR5 in the innate immune response.

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

TLR5 antibody can be used for detection of TLR5 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.

Western blot analysis of TLR5 expression in HT29 cell lysate with TLR5 antibody at (A) 1 and (B) 2 μg/ml.

Immunocytochemistry of TLR5 in THP-1 cells with TLR5 antibody at 10 μg/mL.

Immunofluorescence of TLR5 in THP1 cells with TLR5 antibody at 10 μg/mL.

97,834 Da

toll-like receptor 5

toll-like receptor 5; toll/interleukin-1 receptor-like protein 3; systemic lupus erythematosus susceptibility 1

Toll-like receptor 5

TIL3??[Similar Products]

TLR5_HUMAN

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: plasma membrane; integral to membrane

Molecular Function: interleukin-1 receptor binding; transmembrane receptor activity

Biological Process: positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; male gonad development; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; inflammatory response; positive regulation of toll-like receptor signaling pathway; toll-like receptor 10 signaling pathway

Disease: Legionnaire Disease, Susceptibility To; Systemic Lupus Erythematosus, Susceptibility To, 1; Melioidosis, Susceptibility To

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