Product Name
MED13L, siRNA
Full Product Name
MED13L siRNA (Human)
Product Synonym Names
KIAA1025; PROSIT240; THRAP2; TRAP240L; Mediator of RNA polymerase II transcription subunit 13-like; Mediator complex subunit 13-like; Thyroid hormone receptor-associated protein 2; Thyroid hormone receptor-associated protein complex 240 kDa component-like
Product Gene Name
MED13L sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q71F56
Specificity
MED13L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MED13L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MED13L sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MED13L sirna
siRNA to inhibit MED13L expression using RNA interference
Applications Tested/Suitable for MED13L sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MED13L. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056150.1
[Other Products]
NCBI GenBank Nucleotide #
NM_015335.4
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UniProt Primary Accession #
Q71F56
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UniProt Secondary Accession #
Q68DN4; Q9H8C0; Q9NSY9; Q9UFD8; Q9UPX5; A1L469[Other Products]
UniProt Related Accession #
Q71F56[Other Products]
Molecular Weight
242,602 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 13-like
NCBI Official Synonym Full Names
mediator complex subunit 13-like
NCBI Official Symbol
MED13L??[Similar Products]
NCBI Official Synonym Symbols
THRAP2; TRAP240L; PROSIT240
??[Similar Products]
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 13-like
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 13-like
UniProt Synonym Protein Names
Mediator complex subunit 13-like; Thyroid hormone receptor-associated protein 2; Thyroid hormone receptor-associated protein complex 240 kDa component-like
Protein Family
Mediator of RNA polymerase II transcription
UniProt Gene Name
MED13L??[Similar Products]
UniProt Synonym Gene Names
KIAA1025; PROSIT240; THRAP2; TRAP240L??[Similar Products]
UniProt Entry Name
MD13L_HUMAN
NCBI Summary for MED13L
The protein encoded by this gene is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. The encoded protein is involved in early development of the heart and brain. Defects in this gene are a cause of transposition of the great arteries, dextro-looped (DTGA).[provided by RefSeq, Jul 2010]
UniProt Comments for MED13L
THRAP2: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Defects in MED13L are a cause of transposition of the great arteries dextro-looped type 1 (DTGA1). A congenital heart defect consisting of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required. A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro- looped and mental retardation. Translocation t(12;17)(q24.1;q21). Belongs to the Mediator complex subunit 13 family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 12q24.21
Cellular Component: Srb-mediator complex
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter
Disease: Transposition Of The Great Arteries, Dextro-looped 1
Research Articles on MED13L
1. A meta-analysis of genome-wide association studies of blood pressure and hypertension in Chinese identified three new loci (CACNA1D, CYP21A2, and MED13L) and a newly discovered variant near SLC4A7.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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