For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

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Small volumes of MED13L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7205758 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mediator of RNA polymerase II transcription subunit 13-like (MED13L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MED13L. The ELISA analytical biochemical technique of the MBS7205758 kit is based on MED13L antibody-MED13L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MED13L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MED13L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

242,602 Da

mediator complex subunit 13-like

mediator of RNA polymerase II transcription subunit 13-like; thyroid hormone receptor-associated protein 2; thyroid hormone receptor-associated protein complex 240 kDa component-like

Mediator of RNA polymerase II transcription subunit 13-like

KIAA1025; PROSIT240; THRAP2; TRAP240L??[Similar Products]

MD13L_HUMAN

The protein encoded by this gene is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. The encoded protein is involved in early development of the heart and brain. Defects in this gene are a cause of transposition of the great arteries, dextro-looped (DTGA).[provided by RefSeq, Jul 2010]

THRAP2: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Defects in MED13L are a cause of transposition of the great arteries dextro-looped type 1 (DTGA1). A congenital heart defect consisting of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required. A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro- looped and mental retardation. Translocation t(12;17)(q24.1;q21). Belongs to the Mediator complex subunit 13 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q24.21

Cellular Component: Srb-mediator complex

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Transposition Of The Great Arteries, Dextro-looped 1

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