Anti -WTX, NT (Protein FAM123B, Wilms Tumor Gene On The X Chromosome Protein, FAM123B)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000023.10 (63404997..63425624, complement). Location: Xq11.2

Monoclonal

IgG

D38E5

Rabbit

Recognizes transfected levels of total human WTX.

Supernatant
Supernatant

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-AMER1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

WTX (Wilms' tumor gene on the X chromosome) is a developmentally regulated gene with a potentially important role in kidney development (1). Functional studies of WTX suggest that it acts as a tumor suppressor gene in renal cells by promoting B-catenin ubiquitination and degradation, thereby antagonizing WNT/B-catenin signaling (1,2). WTX is found to be inactivated in 30% of Wilms' tumors, mostly by chromosomal deletion (3). Wilms' tumor is a pediatric kidney cancer that arises from cells that fail to differentiate during kidney development (4). Inactivation of the WT1 tumor suppressor gene accounts for 10-15% of Wilms' tumor cases (5). WTX has been shown to enhance WT1-mediated transactivation, suggesting a physiologically significant interaction between WT1 and WTX (6). WTX may be directly involved in the transcriptional regulation of cellular differentiation in the kidney through interactions with WT1 and other transcription factors (6).

Antibodies; Abs to Cancer Markers

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

124,029 Da[Similar Products]

APC membrane recruitment protein 1

APC membrane recruitment protein 1; protein FAM123B; Wilms tumor on the X; family with sequence similarity 123B; Wilms tumor gene on the X chromosome protein; adenomatous polyposis coli membrane recruitment 1

APC membrane recruitment protein 1

FAM123B; WTX; Amer1??[Similar Products]

AMER1_HUMAN

The protein encoded by this gene upregulates trancriptional activation by the Wilms tumor protein and interacts with many other proteins, including CTNNB1, APC, AXIN1, and AXIN2. Defects in this gene are a cause of osteopathia striata with cranial sclerosis (OSCS). [provided by RefSeq, May 2010]

WTX: a peripheral membrane protein on the cytoplasmic side that shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Enhances trancription activation by WT1. Promotes CTNNB1 ubiquitination and degradation. Antagonizes Wnt and CTNNB1 signaling. Inactivated in approximately one-third of Wilms tumors. Detected in fetal and ***** kidney, brain and spleen. Involved in kidney development. Interacts with CTNNB1, AXIN1, KEAP1, APC and BTRC. Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes containing BTRC and/or FBXW11. Identified in a complex containing CTNNB1, APC, AXIN1 and AXIN2. Two alternatively spliced human isoforms have been reported.

Chromosomal Location of Human Ortholog: Xq11.2

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; beta-catenin binding

Biological Process: Wnt receptor signaling pathway

Disease: Osteopathia Striata With Cranial Sclerosis

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