For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 12; NC_000012.11 (115108059..115121969, complement). Location: 12q24.21

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against TBX3 conjugated to biotin. It has been selected for its ability to recognize TBX3 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-TBX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Biotin Antibody

Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: TBX3 knockout HepG2 cell lysate; Predicted MW: 79,77,65kd Observed MW: 70kd Primary Ab: 2ug/ml Rabbit Anti-Human TBX3 Antibody Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

79,389 Da

T-box 3

T-box transcription factor TBX3; T-box protein 3; bladder cancer related protein XHL

T-box transcription factor TBX3

T-box protein 3??[Similar Products]

TBX3_HUMAN

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

TBX3: Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS). UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.21

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: embryonic forelimb morphogenesis; positive regulation of transcription, DNA-dependent; limbic system development; negative regulation of epithelial cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; female genitalia development; forelimb morphogenesis; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; male genitalia development; heart looping; skeletal development; luteinizing hormone secretion; specification of organ position; blood vessel development; transcription, DNA-dependent; in utero embryonic development; stem cell maintenance; cell aging; positive regulation of cell cycle; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; mesoderm morphogenesis; embryonic digit morphogenesis; follicle-stimulating hormone secretion; negative regulation of myoblast differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Ulnar-mammary Syndrome

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