Mouse Anti-Human CD55 - Purified, Unlabeled Antibody; Mouse Anti-Human CD55

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (207494817..207534311). Location: 1q32

Monoclonal

Mouse IgG1

143-30

Mouse

CD55
CD55, also known as decay accelerating factor (DAF), is a 64-73 kDa GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum, including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters.1-5

Purified (UNLB) Antibody

The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The biotin-maleimide (BIMA) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Small volumes of anti-CD55 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Flow Cytometry; Immunohistochemistry (Frozen Sections)

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

CD55 molecule, decay accelerating factor for complement (Cromer blood group)

complement decay-accelerating factor; CD55 antigen; OTTHUMP00000034398; OTTHUMP00000034399; OTTHUMP00000034400; OTTHUMP00000034420; OTTHUMP00000228537; OTTHUMP00000228538; decay accelerating factor for complement

Complement decay-accelerating factor

DAF_HUMAN

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; extracellular region; plasma membrane; lipid raft

Molecular Function: viral receptor activity; protein binding; lipid binding

Biological Process: respiratory burst; elevation of cytosolic calcium ion concentration; entry of virus into host cell; negative regulation of complement activation; regulation of complement activation; innate immune response; complement activation, classical pathway; regulation of lipopolysaccharide-mediated signaling pathway

Disease: Blood Group, Cromer System

1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD55 Section, Academic Press, New York, p. 281.
3. Lublin, D.M., and J.P. Atkinson. 1989. Annu. Rev. Immunol. 7:35.
4. Holmes, C.H., K.L. Simpson, S.D. Wainwright, C.G. Tate, J.M. Houlihan, I.H. Sawyer, I.P. Rogers, F.A. Spring, D.J. Anstee, and M.J. Tanner. 1990. J. Immunol. 144:3099.
5. Liszewski, M.K., T.C. Farries, D.M. Lublin, I.A. Rooney, and J.P. Atkinson. 1996. Adv. Immunol. 61:201.

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