Product Name
ITGB3, siRNA
Full Product Name
ITGB3 siRNA (Human)
Product Synonym Names
GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61
Product Gene Name
ITGB3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P05106
Specificity
ITGB3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ITGB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ITGB3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ITGB3 sirna
siRNA to inhibit ITGB3 expression using RNA interference
Applications Tested/Suitable for ITGB3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ITGB3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000203.2
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NCBI GenBank Nucleotide #
NM_000212.2
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UniProt Primary Accession #
P05106
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UniProt Secondary Accession #
O15495; Q12806; Q13413; Q14648; Q16499; A0PJW2; D3DXJ8[Other Products]
UniProt Related Accession #
P05106[Other Products]
Molecular Weight
86,694 Da
NCBI Official Full Name
integrin beta-3
NCBI Official Synonym Full Names
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
NCBI Official Symbol
ITGB3??[Similar Products]
NCBI Official Synonym Symbols
GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
??[Similar Products]
NCBI Protein Information
integrin beta-3
UniProt Protein Name
Integrin beta-3
UniProt Synonym Protein Names
Platelet membrane glycoprotein IIIa; GPIIIa; CD_antigen: CD61
UniProt Gene Name
ITGB3??[Similar Products]
UniProt Synonym Gene Names
GP3A; GPIIIa??[Similar Products]
UniProt Entry Name
ITB3_HUMAN
NCBI Summary for ITGB3
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
UniProt Comments for ITGB3
ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: filopodium membrane; platelet alpha granule membrane; cell surface; focal adhesion; microvillus membrane; integral to plasma membrane; melanosome; plasma membrane; nucleus; integrin complex; receptor complex
Molecular Function: identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; protein disulfide isomerase activity; platelet-derived growth factor receptor binding; receptor activity; cell adhesion molecule binding
Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; wound healing; protein folding; cell-matrix adhesion; smooth muscle cell migration; negative chemotaxis; mesodermal cell differentiation; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell adhesion; cell growth; integrin-mediated signaling pathway; platelet activation; regulation of bone resorption; cell migration; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; tube development; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration
Disease: Glanzmann Thrombasthenia; Myocardial Infarction, Susceptibility To; Bleeding Disorder, Platelet-type, 16
Research Articles on ITGB3
1. these results highlight ECO/siITGB3 nanoparticles as a promising therapeutic regimen to combat Triple-negative breast cancer
Precautions
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Disclaimer
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