T-box transcription factor TBX3; T-box protein 3; Tbx3

For Research Use Only. Not for use in diagnostic procedures.

Recombinant and natural Mouse T-box transcription factor TBX3,T-box protein 3

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of Tbx3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2886293 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the T-box transcription factor TBX3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tbx3. The ELISA analytical biochemical technique of the MBS2886293 kit is based on Tbx3 antibody-Tbx3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tbx3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tbx3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

76,949 Da

T-box 3

T-box transcription factor TBX3

T-box transcription factor TBX3

T-box protein 3??[Similar Products]

TBX3_MOUSE

TBX3: Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS). UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleus; transcription factor complex

Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: blood vessel development; cardiac muscle cell differentiation; cell aging; determination of anterior/posterior axis, embryo; embryonic digit morphogenesis; embryonic forelimb morphogenesis; embryonic heart tube development; embryonic hindlimb morphogenesis; female genitalia development; follicle-stimulating hormone secretion; forelimb morphogenesis; heart looping; heart morphogenesis; in utero embryonic development; limb morphogenesis; limbic system development; luteinizing hormone secretion; male genitalia development; mammary gland development; mesoderm morphogenesis; multicellular organismal development; negative regulation of apoptosis; negative regulation of epithelial cell differentiation; negative regulation of myoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ morphogenesis; palate development; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of transcription, DNA-dependent; regulation of cell proliferation; regulation of transcription, DNA-dependent; skeletal development; specification of organ position; stem cell maintenance; transcription, DNA-dependent

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