1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

8D2B8

Mouse

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer

Small volumes of anti-CD59 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Entrez Summary: This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Western blot analysis using CD59 mAb against human CD59 recombinant protein. (Expected MW is 34.7 kDa)

Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA

Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green). Blue

Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue

Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD59 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.

14.2

CD59 molecule

CD59 glycoprotein

CD59 glycoprotein

MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL??[Similar Products]

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: anchored to external side of plasma membrane; cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; focal adhesion; Golgi membrane; membrane; plasma membrane; vesicle

Molecular Function: complement binding; protein binding

Biological Process: blood coagulation; cell activation; cell surface receptor linked signal transduction; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; regulation of complement activation

Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy

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