LL22NC03-44A4.1; YWHA1; 14-3-3 eta

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat YWHAH. No significant cross-reactivity or interference between Rat YWHAH and analogues was observed.

Store at 2-8 degree C.

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Small volumes of YWHAH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286612 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 14-3-3 protein eta (YWHAH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YWHAH. The ELISA analytical biochemical technique of the MBS286612 kit is based on YWHAH antibody-YWHAH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YWHAH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YWHAH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: This assay employs a two-site sandwich ELISA to quantitate YWHAH in samples. An antibody specific for YWHAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YWHAh product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.The 1,730-nucleotide sequence of the cDNA contained a 191-bp 5-prime noncoding region, the complete 738-bp coding region, and an 801-bp 3-prime noncoding region with 3 canonical polyadenylation signals. The eta chain encoded by the cDNA is a 246-amino acid polypeptide with a predicted molecular weight of 28,196.

28,212 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta

14-3-3 protein eta

14-3-3 protein eta

belongs to the 14-3-3 family of proteins; mediates signal transduction via activation of protein kinase C and calcium/calmodulin-dependent protein kinase II [RGD, Feb 2006]

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 ().

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