Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNAmethyltransferase; O-6-methylguanine-DNA-alkyltransferase; MGMT

For Research Use Only. Not for use in diagnostic procedures.

E Coli

>90% as determined by reducing SDS-PAGE.

Supplied as a 0.2 mum filtered solution of 20mM Tris HCl, 1mM EDTA, pH 8.0.

Store at < -20 degree C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of MGMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Molecular Mass: 23.8 kDa
Actual Protein Molecular Mass: 25 kDa

O-6-methylguanine-DNA methyltransferase

methylated-DNA--protein-cysteine methyltransferase

Methylated-DNA--protein-cysteine methyltransferase

MGMT??[Similar Products]

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

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