Anti-ACTG1 / Gamma Actin Antibody (aa188-215) IHC-plus; ACTG; Actin; gamma 1; BRWS2; Cytoskeletal gamma-actin; DFNA20; Actin; cytoplasmic 2; Gamma-actin; DFNA26; Human ACTG1; Gamma Actin

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Ammonium sulfate precipitation

PBS, 0.09% sodium azide

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Small volumes of anti-ACTG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC-P (1:100 - 1:200), WB (1:1000)

Anti-ACTG1 / Gamma Actin antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Confocal immunofluorescent analysis of ACTG1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometry of K562 cells using ACTG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot of ACTG1 Antibody in K562, HepG2 cell line lysates (35 ug/lane). ACTG1 (arrow) was detected using the purified Pab.

Western blot of ACTG1 (arrow) using rabbit polyclonal ACTG1 Antibody. 293 cell lysates (2 ug/lane) either non-transfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.

41,793 Da

actin, gamma 1

actin, cytoplasmic 2; cytoskeletal gamma-actin; epididymis luminal protein 176

Actin, cytoplasmic 2

ACTG??[Similar Products]

ACTG_HUMAN

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: filamentous actin; extracellular space; cytoskeleton; membrane; myofibril; plasma membrane; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding

Biological Process: axon guidance; retinal homeostasis; intercellular junction assembly and maintenance; ephrin receptor signaling pathway; innate immune response; sarcomere organization; vascular endothelial growth factor receptor signaling pathway; cell motility

Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20

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