For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

2A3

Mouse

Purified
Purified IgG - liquid

IgG concentration 1.0 mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-ACTIN GAMMA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human actin gamma antibody, clone 2A3 recognises cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41.8 kDa cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978). Clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Dugina et al. 2009).

Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB)

Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000

HeLa whole cell lysate stained with Mouse anti Human Actin Gamma (MBS224631) followed by Rabbit F(ab')2 anti Mouse IgG:DyLight^?800 (MBS224266). Visualised using the LiCOR Odyssey ^? imager

41,793 Da

actin gamma 1

actin, cytoplasmic 2; cytoskeletal gamma-actin; epididymis luminal protein 176

Actin, cytoplasmic 2

ACTG??[Similar Products]

ACTG_HUMAN

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding

Biological Process: intercellular junction assembly and maintenance; axon guidance; retinal homeostasis; ephrin receptor signaling pathway; innate immune response; sarcomere organization; vascular endothelial growth factor receptor signaling pathway; cell motility

Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20

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