Anti -Myosin IIA, Non-muscle (Cellular Myosin Heavy Chain Type A, DFNA17, EPSTS, FTNS, MGC104539, MHA, MYH2A, MYH9, MYHas8, MyHC-2A, MyHC-IIa, MYHSA2, Myosin 9, Myosin Heavy Chain 9, Myosin Heavy Chain 9 Non-muscle, Myosin Heavy Chain Non-muscle IIa, Myosin Hea

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (36677323..36784107, complement). Location: 22q13.1

Polyclonal

IgG

Rabbit

Recognizes human myosin IIA. Species Crossreactivity: rat and canine.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in 0.01M PBS, pH 7.4, 1% BSA, 15mM sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-MYH9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Myosin heavy chain type A is a critical component of the cellular contractile apparatus. It is involved in maintaining cell-cell adhesion in early mammalian embryos and ablation of this protein leads to prenatal lethality. Mutation in the human MYH9 gene lead to MYH9-related hereditary macrothrombocytopenia (HMTC) characterized by a reduced number of blood platelets.

Antibodies; Abs to Myosin

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunoprecipitation.
Dilution: Immunocytochemistry/Immunofluorescence: 1:100
Western Blot: 1:1000. Detects a band at ~200kD. Staining of myosin IIA (nonmuscle) is inhibited by the immunizing peptide. Additional bands may be detected in some preparations.

226,532 Da[Similar Products]

myosin, heavy chain 9, non-muscle

myosin-9; myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; myosin heavy chain, non-muscle IIa; cellular myosin heavy chain, type A; non-muscle myosin heavy polypeptide 9

Myosin-9

NMMHC-A; NMMHC II-a; NMMHC-IIA??[Similar Products]

MYH9_HUMAN

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: signalosome; cortical cytoskeleton; protein complex; myosin II complex; leading edge; contractile ring; immunological synapse; actomyosin; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; stress fiber; plasma membrane; spindle; uropod; integrin complex; neuromuscular junction; nucleus; cleavage furrow

Molecular Function: microfilament motor activity; calmodulin binding; actin filament binding; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; ADP binding; actin binding; actin-dependent ATPase activity; ATP binding

Biological Process: integrin-mediated signaling pathway; axon guidance; blood vessel endothelial cell migration; monocyte differentiation; in utero embryonic development; actin filament-based movement; membrane protein ectodomain proteolysis; actomyosin structure organization and biogenesis; cytokinesis; uropod organization and biogenesis; establishment of meiotic spindle localization; regulation of cell shape; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; angiogenesis; platelet formation; leukocyte migration; meiotic spindle organization and biogenesis; myoblast fusion

Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome

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