For Research Use Only. Not for use in diagnostic procedures.

Store at -20 degree C for 12 months.

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Background/Introduction: Acidic Mammalian Chitinase (AMCase) belongs to the glycosyl hydrolase-18 family (EC 3.2.1.14), which is capable of hydrolyzing the beta-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. AMCase is relatively abundant in the gastrointestinal tract and lung. It is highly expressed in the lungs of asthmatic patients and asthma animal models. In addition, the change in AMCase expression can be used to monitor Th-2/IL-13 mediated immune response and inflammation. Inhibition of AMCase activity has been considered as a therapeutic strategy to alleviate symptoms of allergic airway inflammation. Thus, there is a need to identify AMCase inhibitors as potential candidates to treat and/or prevent asthma. Acidic Mammalian Chitinase Inhibitor Screening Kit uses Bisdionin F? a highly selective AMCase inhibitor as positive control and it provides a simple, rapid, sensitive and reliable test suitable for screening/characterizing AMCase inhibitors.

Protease

Screening/characterizing/studying Mammalian Chitinase inhibitors/activators

33,906 Da[Similar Products]

chitinase, acidic

acidic mammalian chitinase

Acidic mammalian chitinase

AMCase??[Similar Products]

The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

CHIA: Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: chitin binding; chitinase activity; kinase binding

Biological Process: carbohydrate metabolic process; cell wall chitin metabolic process; chitin catabolic process; production of molecular mediator of acute inflammatory response; response to fungus

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