For Research Use Only. Not for use in diagnostic procedures.

Store at -20 degree C for 12 months.

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Background/Introduction: Acidic Mammalian Chitinase (AMCase) and Chitotriosidase-1(CHIT1) are the only two mammalian active chitinases that belong to the glycosyl hydrolase-18 family (EC 3.2.1.14). They are chitin-fragmenting hydrolases that hydrolyze the beta-(1,4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. In contrast to CHIT1, AMCase exhibits its optimum activity under acidic conditions and is relatively abundant in the gastrointestinal tract and lungs. AMCase is found highly expressed both in animal models used for the study of allergic inflammation and in lungs of human asthma. Recent data suggests a major biological function of AMCase in asthma and the changes in AMCase expression can be used to monitor Th-2/IL-13-mediated immune response and inflammation. Acidic Mammalian Chitinase Activity Assay Kit utilizes the ability of AMCase to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 320/445 nm) and it uses a specific Lysis Buffer which distinguishes AMCase activity from CHIT1 activity. This kit provides a simple, specific, ultra-sensitive assay that can detect as low as 0.5 mU/ml of AMCase activity in a variety of biological samples.

Carbohydrate Metabolism

Measurement of Chitinase activity in various biological samples and purified/crude enzyme preparations

33,906 Da[Similar Products]

chitinase, acidic

acidic mammalian chitinase

Acidic mammalian chitinase

AMCase??[Similar Products]

The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

CHIA: Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 3.2.1.14; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: chitin binding; chitinase activity; kinase binding

Biological Process: carbohydrate metabolic process; cell wall chitin metabolic process; chitin catabolic process; production of molecular mediator of acute inflammatory response; response to fungus

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