Product Name
PKD mu, Polyclonal Antibody
Full Product Name
PKD mu antibody
Product Synonym Names
Polyclonal PKD mu; Anti-PKD mu; Ab 910
Product Gene Name
anti-PKD mu antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15139
Species Reactivity
Human, Mouse, Rat
Purity/Purification
PKD mu antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Biological Significance
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKC
Immunogen
PKD mu antibody was raised in Rabbit using the peptide sequence around aa 908~912 (R-V-S-I-L) derived from Human PKD/PKCmu as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-PKD mu antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PKD mu antibody
Rabbit polyclonal PKD mu antibody
Product Categories/Family for anti-PKD mu antibody
Cell Biology
Applications Tested/Suitable for anti-PKD mu antibody
Western Blot (WB)
Application Notes for anti-PKD mu antibody
WB: 1:500-1:1000
Western Blot (WB) of anti-PKD mu antibody
Western Blot analysis of extracts from Hela, 293 and 3T3 cells using PKD/PKCu antibody
NCBI/Uniprot data below describe general gene information for PKD mu. It may not necessarily be applicable to this product.
NCBI Accession #
CAA53384.1
[Other Products]
UniProt Primary Accession #
Q15139
[Other Products]
UniProt Secondary Accession #
A6NL64; B2RAF6[Other Products]
UniProt Related Accession #
Q15139[Other Products]
Molecular Weight
101,704 Da
NCBI Official Full Name
protein kinase C mu
NCBI Official Synonym Full Names
protein kinase D1
NCBI Official Symbol
PRKD1??[Similar Products]
NCBI Official Synonym Symbols
PKD; PKCM; PRKCM; PKC-MU
??[Similar Products]
NCBI Protein Information
serine/threonine-protein kinase D1
UniProt Protein Name
Serine/threonine-protein kinase D1
UniProt Synonym Protein Names
Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
UniProt Gene Name
PRKD1??[Similar Products]
UniProt Synonym Gene Names
PKD; PKD1; PRKCM??[Similar Products]
UniProt Entry Name
KPCD1_HUMAN
NCBI Summary for PKD mu
PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010]
UniProt Comments for PKD mu
PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A
Protein type: Protein kinase, CAMK; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Autophagy; Kinase, protein; Motility/polarity/chemotaxis; CAMK group; PKD family
Chromosomal Location of Human Ortholog: 14q11
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; cell cortex; intercellular junction; trans-Golgi network; nucleus; cytosol
Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding
Biological Process: integrin-mediated signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid metabolic process; sphingolipid biosynthetic process; apoptosis; protein amino acid autophosphorylation; negative regulation of endocytosis; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; signal transduction; positive regulation of peptidyl-serine phosphorylation; Golgi vesicle transport; activation of NF-kappaB transcription factor; cell proliferation; positive regulation of osteoblast differentiation; peptidyl-serine phosphorylation; positive regulation of angiogenesis; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; inflammatory response; vascular endothelial growth factor receptor signaling pathway; Golgi organization and biogenesis
Research Articles on PKD mu
1. a positive relationship between L1 and pPKD1 in both cultured cerebellar neurons and human cerebellar tissue, suggesting that L1 functions in the modulation of PKD1 phosphorylation.
Precautions
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Disclaimer
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