Anti -Myosin IIA, Non-muscle, Phosphorylated (Ser1943) (Myosin-9, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-Muscle IIa, Non-Muscle Myosin Heavy Chain IIa, NMMHC II-a, NMMHC-IIA, Cellular Myosin Heavy Chain, Type A, Non-Muscle Myosin Heavy Chain A, NMMHC-A,

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (36677323..36784107, complement). Location: 22q13.1

Polyclonal

Rabbit

Recognizes human Myosin Heay Chain 2A phosphorylated at Ser1943. Species sequence homology: Rhesus macaque, canine (91%), porcine (90%), rat and mouse (80%).

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-MYH9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Myosin-2A heavy chain (MYH9) phosphorylation is known to be very important in regulating cell motility and chemotaxis. The suppression of myosin-2A heavy chain expression is strongly associated with osteoclast fusion and multinucleation. Recent research indicates that modifications in the myosin-IIA heavy chain could increase the risk of kidney disease. Myosin-2A heavy chain is shown to work with WAVE2 and is localized at the leading edge of the lamellipodia formation in cells.

Antibodies; Abs to Myosin

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence.
Dilution: Western Blot: 0.1ug/ml
Immunocytochemistry: 1:500
Immunofluorescence: 1:400

226,532 Da[Similar Products]

myosin, heavy chain 9, non-muscle

myosin-9; myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; myosin heavy chain, non-muscle IIa; cellular myosin heavy chain, type A; non-muscle myosin heavy polypeptide 9

Myosin-9

NMMHC-A; NMMHC II-a; NMMHC-IIA??[Similar Products]

MYH9_HUMAN

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: signalosome; cortical cytoskeleton; protein complex; myosin II complex; leading edge; contractile ring; actomyosin; immunological synapse; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; plasma membrane; stress fiber; spindle; uropod; neuromuscular junction; nucleus; integrin complex; cleavage furrow

Molecular Function: calmodulin binding; actin filament binding; microfilament motor activity; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; actin-dependent ATPase activity; ADP binding; actin binding; ATP binding

Biological Process: integrin-mediated signaling pathway; axon guidance; blood vessel endothelial cell migration; monocyte differentiation; actin filament-based movement; in utero embryonic development; membrane protein ectodomain proteolysis; actomyosin structure organization and biogenesis; cytokinesis; uropod organization and biogenesis; regulation of cell shape; establishment of meiotic spindle localization; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; platelet formation; angiogenesis; leukocyte migration; meiotic spindle organization and biogenesis; myoblast fusion

Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome

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