Anti -Myosin IIA, non-muscle, heavy chain (Recombinant) (Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 22; NC_000022.10 (36677323..36784107, complement). Location: 22q13.1

Monoclonal

IgG2,l

SF9

Human

Recognizes rat myosin IAA (heavy chain).

Highly Purified
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).

Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

1 mg/ml (lot specific)

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of MYH9 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Antibodies; Abs to Myosin

Suitable for use in Electron Microscopy, ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.

Immunocytochemistry: 1:1000
Western Blot: 1:1000

Optimal dilutions to be determined by researcher.

Schematic antibody structure

Immunofluorescence/Immunocytochemistry analysis of human Myosin IIA (non-muscle) (heavy chain) using MBS637834.

Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with MBS637834 (1ug /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.

myosin, heavy chain 9, non-muscle

myosin-9; myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; myosin heavy chain, non-muscle IIa; cellular myosin heavy chain, type A; non-muscle myosin heavy polypeptide 9

Myosin-9

NMMHC-A; NMMHC II-a; NMMHC-IIA??[Similar Products]

MYH9_HUMAN

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cortical cytoskeleton; signalosome; protein complex; myosin II complex; leading edge; contractile ring; actomyosin; immunological synapse; cytosol; actin cytoskeleton; ruffle; cell-cell adherens junction; membrane; cytoplasm; stress fiber; plasma membrane; spindle; uropod; neuromuscular junction; integrin complex; nucleus; cleavage furrow

Molecular Function: actin filament binding; microfilament motor activity; calmodulin binding; protein binding; protein homodimerization activity; protein anchor; ATPase activity; motor activity; ADP binding; actin-dependent ATPase activity; actin binding; ATP binding

Biological Process: integrin-mediated signaling pathway; axon guidance; monocyte differentiation; blood vessel endothelial cell migration; in utero embryonic development; actin filament-based movement; actomyosin structure organization and biogenesis; membrane protein ectodomain proteolysis; cytokinesis; uropod organization and biogenesis; establishment of meiotic spindle localization; regulation of cell shape; protein transport; actin cytoskeleton reorganization; ephrin receptor signaling pathway; establishment of T cell polarity; platelet formation; angiogenesis; leukocyte migration; meiotic spindle organization and biogenesis; myoblast fusion

Disease: Deafness, Autosomal Dominant 17; May-hegglin Anomaly; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; Epstein Syndrome; Sebastian Syndrome

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