BTK, AGMX1, ATK, BPK

For Research Use Only. Not for use in diagnostic procedures.

Baculovirus-Insect Cells

> 85 % as determined by SDS-PAGE

Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0

Samples are stable for up to twelve months from date of receipt at -70 degree C

Manufactured in an ISO 9001:2015 Certified Laboratory.

Small volumes of BTK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol (3, 4, 5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia (Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.

Description: A DNA sequence encoding the human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag.

The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 KDa.

79,937 Da

Bruton tyrosine kinase

tyrosine-protein kinase BTK

Tyrosine-protein kinase BTK

AGMX1; ATK; BPK; ATK; BPK??[Similar Products]

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; nucleus; plasma membrane

Molecular Function: ATP binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; B cell activation; B cell receptor signaling pathway; calcium-mediated signaling; innate immune response; mesoderm development; MyD88-dependent toll-like receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of B cell differentiation; protein amino acid phosphorylation; regulation of B cell apoptosis; regulation of B cell cytokine production; regulation of cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Agammaglobulinemia, X-linked; Isolated Growth Hormone Deficiency, Type Iii

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.