Anti-SOX3 Antibody (C-Terminus) IHC-plus; SOX3; GHDX; MRGH; SOXB; Panhypopituitarism; PHPX; Sry-box3; PHP; Transcription factor SOX-3; Human SOX3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Human SOX3.

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-SOX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Family: Transcription factor

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA (1:8000), IHC-P (3.75 ug/ml), WB (0.5 - 1.5 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 42kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 45.2kDa according to NP_005625.2). Recommended concentration:...

Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

SOX3 antibody (0.5 ug/ml) staining of Human Cerebral Cortex lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45,210 Da

SRY (sex determining region Y)-box 3

transcription factor SOX-3

Transcription factor SOX-3

SOX3_HUMAN

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]

SOX3: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Defects in SOX3 are a cause of panhypopituitarism X- linked (PHPX). Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X- linked with isolated growth hormone deficiency (MRXGH). Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3). A condition in which male gonads develop in a genetic female (female to male sex reversal). Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: sex determination; negative regulation of neuron differentiation; spermatid differentiation; hypothalamus development; organ morphogenesis; central nervous system development; sensory organ development; transcription, DNA-dependent; pituitary gland development; neuron development; negative regulation of transcription from RNA polymerase II promoter; Sertoli cell development

Disease: Panhypopituitarism, X-linked; Mental Retardation, X-linked, With Panhypopituitarism

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.