Product Name
Tumor Necrosis Factor Alpha (TNFa), Polyclonal Antibody
Popular Item
Full Product Name
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Product Gene Name
anti-TNFa antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for http://www.ncbi.nlm.nih.gov/protein/AEW10516.1
Species Reactivity
Chicken
Specificity
The antibody is a?Rabbit polyclonal?antibody raised against?TNFa. It has been selected for its ability to recognize?TNFa in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
200ug/ml (lot specific)
Source
Polyclonal antibody preparation
Immunogen
Recombinant TNFa (Arg1~Phe73) expressed in E.coli
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038459
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of anti-TNFa antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TNFa antibody
WB; IHC; ICC; IP
Application Notes for anti-TNFa antibody
Western blotting: 0.5-2μg/mL
Immunocytochemistry in formalin fixed cells: 5-20μg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20μg/mL
Immunohistochemistry in paraffin section: 5-20μg/mL
Optimal working dilutions must be determined by end user
Western Blot (WB) of anti-TNFa antibody
Western Blot: Sample: Recombinant protein.

NCBI/Uniprot data below describe general gene information for TNFa. It may not necessarily be applicable to this product.
NCBI Accession #
AEW10516.1
[Other Products]
UniProt Primary Accession #
http://www.ncbi.nlm.nih.gov/protein/AEW10516.1
[Other Products]
UniProt Related Accession #
Q8QGW7[Other Products]
Molecular Weight
16,013 Da
NCBI Official Full Name
tumor necrosis factor-alpha, partial
NCBI Official Synonym Full Names
lipopolysaccharide induced TNF factor
NCBI Official Symbol
LITAF??[Similar Products]
NCBI Official Synonym Symbols
TNF-alpha
??[Similar Products]
NCBI Protein Information
lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
UniProt Protein Name
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
UniProt Synonym Protein Names
Small integral membrane protein of lysosome/late endosome
Protein Family
Tumor necrosis factor alpha-induced protein
UniProt Gene Name
LITAF??[Similar Products]
UniProt Synonym Gene Names
SIMPLE; LPS-induced TNF-alpha factor homolog??[Similar Products]
UniProt Comments for TNFa
Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role in the nucleus in regulating the expression of numerous cytokines.
Research Articles on TNFa
1. The results showed that Se deficiency induced high expression levels of PTGE, COX-2, TNF-alpha, and NF-kappaB in the gastrointestinal tract tissues.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.