Proline dehydrogenase 1; mitochondrial; Proline oxidase; Prodh; Pro1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-Prodh antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

PRODH is a mitochondrial proline dehydrogenase that catalyzes the first step in proline degradation. It converts proline to delta-1-pyrroline-5-carboxylate.

Cancer; Cell Biology; Signal Transduction

Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), ELISA (EIA)

FC~~1:25
IHC-P~~1:50~100
WB~~1:1000

Overlay histogram showing A549 cells stainedwith MBS9207635 (green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9207635, 1:25 dilution) for 60 min at 37oC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight? 488Conjugated Highly Cross-Adsorbed(OH191631)at 1/200 dilution for 40 min at 37oC. Isotypecontrol antibody (blue line) was rabbit IgG(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

MBS9207635_IHC staining PRODH in H. skeletal musclesections by Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.

Anti-PRODH Antibody (Center) at 1: 2000 dilution + MDA-MB-468 whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5%NFDM/TBST.

Anti-PRODH Antibody (Center) at 1:2000dilution + human brain lysate Lysates/proteinsat 20 μg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-PRODH Antibody (Center) at1:1000 dilution Lane 1: MDA-MB-468 whole celllysates Lane 2: A549 whole cell lysatesLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of mouse Prodh Antibody (Center) in mouse liver, kidney tissue lysates (35ug/lane). PRODH (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

proline dehydrogenase

proline dehydrogenase 1, mitochondrial

Proline dehydrogenase 1, mitochondrial

Pro1??[Similar Products]

PROD_MOUSE

PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Mitochondrial; EC 1.5.5.2; Amino Acid Metabolism - arginine and proline

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: amino acid binding; proline dehydrogenase activity; oxidoreductase activity

Biological Process: proline catabolic process; proline metabolic process

Polyak,K., et.al., Nature 389 (6648), 300-305 (1997) Gogos,J.A., et.al., Nat. Genet. 21 (4), 434-439 (1999)

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