Product Name
PRKD1, siRNA
Full Product Name
PRKD1 siRNA (Mouse)
Product Synonym Names
PKCM; PKD; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
Product Gene Name
PRKD1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62101
Specificity
PRKD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PRKD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PRKD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PRKD1 sirna
siRNA to inhibit PRKD1 expression using RNA interference
Applications Tested/Suitable for PRKD1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PRKD1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032884.2
[Other Products]
NCBI GenBank Nucleotide #
NM_008858.3
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UniProt Primary Accession #
Q62101
[Other Products]
UniProt Secondary Accession #
E9QNA3[Other Products]
UniProt Related Accession #
Q62101[Other Products]
Molecular Weight
102,037 Da
NCBI Official Full Name
serine/threonine-protein kinase D1
NCBI Official Synonym Full Names
protein kinase D1
NCBI Official Symbol
Prkd1??[Similar Products]
NCBI Official Synonym Symbols
PKD; PKD1; Pkcm; Prkcm
??[Similar Products]
NCBI Protein Information
serine/threonine-protein kinase D1
UniProt Protein Name
Serine/threonine-protein kinase D1
UniProt Synonym Protein Names
Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
Prkd1??[Similar Products]
UniProt Synonym Gene Names
Pkcm; Pkd; Prkcm??[Similar Products]
UniProt Entry Name
KPCD1_MOUSE
UniProt Comments for PRKD1
PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A
Protein type: Motility/polarity/chemotaxis; EC 2.7.11.13; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Autophagy; Kinase, protein; CAMK group; PKD family
Cellular Component: Golgi apparatus; membrane; cell; cytoplasm; plasma membrane; intercellular junction; trans-Golgi network; cell cortex; intracellular; cytosol; nucleus
Molecular Function: transferase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; protein kinase C activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: nervous system development; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; apoptosis; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of release of sequestered calcium ion into cytosol; activation of NF-kappaB transcription factor; Golgi vesicle transport; positive regulation of osteoblast differentiation; peptidyl-serine phosphorylation; positive regulation of angiogenesis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell differentiation; inflammatory response; phosphorylation; Golgi organization and biogenesis
Research Articles on PRKD1
1. PKD1-deficient keratinocytes showed an increase in transglutaminase expression and activity, indicating an anti-differentiative role of PKD1. Furthermore, the PKD1-deficient keratinocytes exhibited decreased proliferation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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