Anti-CARD9 Antibody (aa521-536) IHC-plus; CARD9; CANDF2; HCARD9; Human CARD9

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Synthetic peptide (DRENTTGSDNTDTEGS) corresponding to amino acids 521 to 536 of human CARD9 The sequence is different from that of rat origin by two amino acids.

Immunoaffinity Purified

PBS, 0.02% sodium azide.

1 mg/ml (lot specific)

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Small volumes of anti-CARD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10.

Family: Apoptosis

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)

ICC, IHC-P (10 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Anti-CARD9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 ug/ml.

Western blot of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 ug /ml.

42,953 Da

caspase recruitment domain family, member 9

caspase recruitment domain-containing protein 9

Caspase recruitment domain-containing protein 9

hCARD9??[Similar Products]

CARD9_HUMAN

The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]

CARD9: Activates NF-kappa-B via BCL10. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2). Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytoplasm

Molecular Function: protein homodimerization activity; CARD domain binding

Biological Process: response to drug; response to fungus; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of interleukin-2 biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of tumor necrosis factor production; positive regulation of stress-activated MAPK cascade; regulation of apoptosis; defense response to Gram-positive bacterium; response to exogenous dsRNA; response to muramyl dipeptide; response to peptidoglycan; innate immune response; defense response to virus; regulation of tumor necrosis factor biosynthetic process

Disease: Candidiasis, Familial, 2

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