Product Name
SLC4A11, siRNA
Full Product Name
SLC4A11 siRNA (Mouse)
Product Synonym Names
BTR1; Sodium bicarbonate transporter-like protein 11; Bicarbonate transporter-related protein 1; Sodium borate cotransporter 1; NaBC1; Solute carrier family 4 member 11
Product Gene Name
SLC4A11 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A2AJN7
Specificity
SLC4A11 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC4A11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC4A11 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC4A11 sirna
siRNA to inhibit SLC4A11 expression using RNA interference
Applications Tested/Suitable for SLC4A11 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC4A11. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074631.1
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NCBI GenBank Nucleotide #
NM_001081162.1
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UniProt Primary Accession #
A2AJN7
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UniProt Secondary Accession #
Q0VG86; Q3URQ1[Other Products]
UniProt Related Accession #
A2AJN7[Other Products]
Molecular Weight
96,751 Da
NCBI Official Full Name
sodium bicarbonate transporter-like protein 11
NCBI Official Synonym Full Names
solute carrier family 4, sodium bicarbonate transporter-like, member 11
NCBI Official Symbol
Slc4a11??[Similar Products]
NCBI Official Synonym Symbols
BTR1; NaBC1; AI503023
??[Similar Products]
NCBI Protein Information
sodium bicarbonate transporter-like protein 11
UniProt Protein Name
Sodium bicarbonate transporter-like protein 11
UniProt Synonym Protein Names
Bicarbonate transporter-related protein 1; Sodium borate cotransporter 1; NaBC1; Solute carrier family 4 member 11
Protein Family
Sodium bicarbonate transporter-like protein
UniProt Gene Name
Slc4a11??[Similar Products]
UniProt Synonym Gene Names
BTR1; NaBC1??[Similar Products]
UniProt Entry Name
S4A11_MOUSE
UniProt Comments for SLC4A11
SLC4A11: Transporter which plays an important role in sodium- mediated fluid transport in different organs. Prevents severe morphological changes of the cornea caused by increased sodium chloride concentrations in the stroma. In the inner ear, is involved in transport of potassium through the fibrocyte layer to the stria vascularis and is essential for the generation of the endocochlear potential but not for regulation of potassium concentrations in the endolymph. In the kidney, is essential for urinary concentration, mediates a sodium flux into the thin descending limb of Henle loop to allow countercurrent multiplication by osmotic equilibration. Involved in borate homeostasis. In the absence of borate, it functions as a Na(+) and OH(-)(H(+)) channel. In the presence of borate functions as an electrogenic Na(+) coupled borate cotransporter. Defects in SLC4A11 are the cause of corneal dystrophy and perceptive deafness (CDPD); also known as corneal dystrophy and sensorineural deafness or Harboyan syndrome. CDPD consists of congenital corneal endothelial dystrophy and progressive perceptive deafness. Inheritance is autosomal recessive. Defects in SLC4A11 are the cause of corneal endothelial dystrophy type 2 (CHED2); also known as congenital hereditary endothelial dystrophy of cornea. This bilateral corneal dystrophy is characterized by corneal opacification and nystagmus. Inheritance is autosomal recessive. Defects in SLC4A11 are the cause of corneal dystrophy Fuchs endothelial type 4 (FECD4); also known as Corneal dystrophy Fuchs endothelial late-onset. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Belongs to the anion exchanger (TC 2.A.31) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: membrane; integral to plasma membrane; cell; integral to membrane; plasma membrane
Molecular Function: protein dimerization activity; bicarbonate transmembrane transporter activity; inorganic anion exchanger activity; transporter activity; symporter activity; anion:anion antiporter activity; boron transporter activity; anion transmembrane transporter activity; hydrogen ion channel activity; sodium channel activity
Biological Process: proton transport; fluid transport; cellular cation homeostasis; ion homeostasis; transport; inorganic anion transport; bicarbonate transport; sodium ion transport; regulation of intracellular pH; ion transport; anion transport; boron transport
Research Articles on SLC4A11
1. the declined tumor cell survival following inhibition of BCT could be the consequence of interplay of several inter-connected regulatory molecular events.
Precautions
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Disclaimer
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