Transcription factor SOX-3; SOX3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This SOX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-196 amino acids from the Central region of human SOX3.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.317 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-SOX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the SOX (SRY-related
HMG-box) family of transcription factors involved in the regulation
of embryonic development and in the determination of the cell fate.
The encoded protein may act as a transcriptional regulator after
forming a protein complex with other proteins. Mutations in this
gene have been associated with X-linked mental retardation with
growth hormone deficiency.

Neuroscience

Western Blot (WB), ELISA (EIA)

WB~~1:1000

SOX3 Antibody (Center) western blot analysis in MDA-MB453,HL-60 cell line lysates (35ug/lane).This demonstrates the SOX3 antibody detected the SOX3 protein (arrow).

45210

SRY (sex determining region Y)-box 3

transcription factor SOX-3

Transcription factor SOX-3

SOX3_HUMAN

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]

SOX3: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Defects in SOX3 are a cause of panhypopituitarism X- linked (PHPX). Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X- linked with isolated growth hormone deficiency (MRXGH). Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3). A condition in which male gonads develop in a genetic female (female to male sex reversal). Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: sex determination; organ morphogenesis; hypothalamus development; negative regulation of neuron differentiation; spermatid differentiation; central nervous system development; sensory organ development; transcription, DNA-dependent; pituitary gland development; neuron development; negative regulation of transcription from RNA polymerase II promoter; Sertoli cell development

Disease: Panhypopituitarism, X-linked; Mental Retardation, X-linked, With Panhypopituitarism

Dateki, S., et al. J. Clin. Endocrinol. Metab. 95(8):4043-4047(2010)
Sato, Y., et al. Mol. Cell. Biochem. 337 (1-2), 267-275 (2010) :
Mojsin, M., et al. Biochem. J. 425(1):107-116(2010)
Bleyl, S.B., et al. Am. J. Med. Genet. A 143A (23), 2785-2795 (2007) :
Krstic, A., et al. Arch. Biochem. Biophys. 467(2):163-173(2007)

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