Product Name
LITAF, siRNA
Full Product Name
LITAF siRNA (Mouse)
Product Synonym Names
N4WBP3; TBX1; Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; LPS-induced TNF-alpha factor homolog; Estrogen-enhanced transcript protein; mEET; LITAF-like protein; NEDD4 WW domain-binding protein 3
Product Gene Name
LITAF sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JLJ0
Specificity
LITAF siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LITAF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LITAF sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LITAF sirna
siRNA to inhibit LITAF expression using RNA interference
Applications Tested/Suitable for LITAF sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LITAF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_064364.1
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NCBI GenBank Nucleotide #
NM_019980.2
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UniProt Primary Accession #
Q9JLJ0
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UniProt Secondary Accession #
Q9EQI0[Other Products]
UniProt Related Accession #
Q9JLJ0[Other Products]
Molecular Weight
16,946 Da
NCBI Official Full Name
lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
NCBI Official Synonym Full Names
LPS-induced TN factor
NCBI Official Symbol
Litaf??[Similar Products]
NCBI Official Synonym Symbols
TBX1; C85531; N4WBP3; 3222402J11Rik
??[Similar Products]
NCBI Protein Information
lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
UniProt Protein Name
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
UniProt Synonym Protein Names
Estrogen-enhanced transcript protein; mEET; LITAF-like protein; NEDD4 WW domain-binding protein 3
Protein Family
Lipopolysaccharide-induced tumor necrosis factor-alpha factor
UniProt Gene Name
Litaf??[Similar Products]
UniProt Synonym Gene Names
N4wbp3; Tbx1; LPS-induced TNF-alpha factor homolog; mEET??[Similar Products]
UniProt Entry Name
LITAF_MOUSE
UniProt Comments for LITAF
LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Oncoprotein
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; membrane; lysosomal membrane; lysosome; cytoplasm; plasma membrane
Molecular Function: signal transducer activity; protein binding; WW domain binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; response to lipopolysaccharide; negative regulation of NF-kappaB import into nucleus; signal transduction; regulation of cytokine production
Research Articles on LITAF
1. Mutation of SIMPLE (Litaf) in Charcot-Marie-Tooth 1C alters production of exosomes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
