Product Name
USP26, siRNA
Full Product Name
USP26 siRNA (Human)
Product Synonym Names
Ubiquitin carboxyl-terminal hydrolase 26; Deubiquitinating enzyme 26; Ubiquitin thioesterase 26; Ubiquitin-specific-processing protease 26
Product Gene Name
USP26 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BXU7
Specificity
USP26 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human USP26 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of USP26 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
USP26 sirna
siRNA to inhibit USP26 expression using RNA interference
Applications Tested/Suitable for USP26 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for USP26. It may not necessarily be applicable to this product.
NCBI Accession #
NP_114113.1
[Other Products]
NCBI GenBank Nucleotide #
NM_031907.1
[Other Products]
UniProt Primary Accession #
Q9BXU7
[Other Products]
UniProt Secondary Accession #
Q5H9H4; B9WRT6[Other Products]
UniProt Related Accession #
Q9BXU7[Other Products]
Molecular Weight
104,047 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 26
NCBI Official Synonym Full Names
ubiquitin specific peptidase 26
NCBI Official Symbol
USP26??[Similar Products]
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 26
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 26
UniProt Synonym Protein Names
Deubiquitinating enzyme 26; Ubiquitin thioesterase 26; Ubiquitin-specific-processing protease 26
UniProt Gene Name
USP26??[Similar Products]
UniProt Entry Name
UBP26_HUMAN
NCBI Summary for USP26
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases and is a deubiquitinating enzyme (DUB) with His and Cys domains. It is specifically expressed in testis tissue. Mutations in this gene have been associated with Sertoli cell-only syndrome and male infertility. [provided by RefSeq, Jul 2008]
UniProt Comments for USP26
USP26: Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome. Deubiquitinates the androgen receptor and regulates the androgen receptor signaling pathway. Belongs to the peptidase C19 family.
Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: Xq26.2
Cellular Component: nucleus
Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination
Disease: Spermatogenic Failure, X-linked, 1
Research Articles on USP26
1. There is an association of alteration in USP26 with male infertility, especially in azoospermia and in the Asian population. [Meta-Analysis]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
