AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA; Bruton Agammaglobulinemia Tyrosine Kinase; B-cell progenitor kinase

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Bruton'S Tyrosine Kinase (Btk).
No significant cross-reactivity or interference between Bruton'S Tyrosine Kinase (Btk) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Btk elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2020613 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bruton'S Tyrosine Kinase (Btk) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Btk. The ELISA analytical biochemical technique of the MBS2020613 kit is based on Btk antibody-Btk antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Btk antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Btk. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bruton'S Tyrosine Kinase (Btk). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bruton'S Tyrosine Kinase (Btk). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bruton'S Tyrosine Kinase (Btk), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bruton'S Tyrosine Kinase (Btk) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Signal transduction; Enzyme & Kinase; Infection immunity; Hematology

79,937 Da

Bruton agammaglobulinemia tyrosine kinase

tyrosine-protein kinase BTK

Tyrosine-protein kinase BTK

AGMX1; ATK; BPK; ATK; BPK??[Similar Products]

BTK_HUMAN

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein-tyrosine kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; B cell activation; transcription, DNA-dependent; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; regulation of cell proliferation; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; positive regulation of B cell differentiation; innate immune response; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Agammaglobulinemia, X-linked; Isolated Growth Hormone Deficiency, Type Iii

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