Full Product Name
JAK2 siRNA (Rat)
Product Synonym Names
Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2
Product Gene Name
JAK2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62689
Specificity
JAK2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat JAK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of JAK2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
JAK2 sirna
siRNA to inhibit JAK2 expression using RNA interference
Applications Tested/Suitable for JAK2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for JAK2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_113702.1
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NCBI GenBank Nucleotide #
NM_031514.1
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UniProt Primary Accession #
Q62689
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UniProt Related Accession #
Q62689[Other Products]
Molecular Weight
130,585 Da
NCBI Official Full Name
tyrosine-protein kinase JAK2
NCBI Official Synonym Full Names
Janus kinase 2
NCBI Official Symbol
Jak2??[Similar Products]
NCBI Protein Information
tyrosine-protein kinase JAK2
UniProt Protein Name
Tyrosine-protein kinase JAK2
UniProt Synonym Protein Names
Janus kinase 2; JAK-2
Protein Family
Tyrosine-protein kinase
UniProt Gene Name
Jak2??[Similar Products]
UniProt Synonym Gene Names
JAK-2??[Similar Products]
UniProt Entry Name
JAK2_RAT
NCBI Summary for JAK2
associated with receptors for erythropoietin, prolactin, growth hormones and interleukin-3; phosphorylated when activated by cytokines [RGD, Feb 2006]
UniProt Comments for JAK2
JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490.
Protein type: Oncoprotein; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; JakA family
Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; nuclear matrix; cytoplasm; endomembrane system; caveola; intracellular; nucleus; cytosol; lipid raft
Molecular Function: protein C-terminus binding; protein binding; growth hormone receptor binding; peptide hormone receptor binding; histone binding; insulin receptor substrate binding; protein-tyrosine kinase activity; heme binding; acetylcholine receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoinositide 3-kinase binding; type 1 angiotensin receptor binding; SH2 domain binding; protein kinase binding; ATP binding; interleukin-12 receptor binding; receptor binding
Biological Process: positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; positive regulation of apoptosis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; tyrosine phosphorylation of JAK2 protein; enzyme linked receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; response to antibiotic; elevation of cytosolic calcium ion concentration; tumor necrosis factor-mediated signaling pathway; erythrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of cell activation; positive regulation of DNA binding; positive regulation of protein import into nucleus, translocation; axon regeneration; positive regulation of insulin secretion; positive regulation of tumor necrosis factor production; JAK-STAT cascade; tyrosine phosphorylation of Stat5 protein; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; mineralocorticoid receptor signaling pathway; response to hydroperoxide; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; response to oxidative stress; positive regulation of cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of apoptosis; positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; hormone-mediated signaling; negative regulation of heart contraction; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; signal transduction; host programmed cell death induced by symbiont; negative regulation of cell proliferation; positive regulation of cell proliferation; cell differentiation; STAT protein nuclear translocation; caspase activation; cytokine and chemokine mediated signaling pathway; negative regulation of DNA binding; G-protein coupled receptor protein signaling pathway; positive regulation of interleukin-1 beta production; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of inflammatory response; myeloid cell differentiation; negative regulation of cell-cell adhesion; induction of apoptosis by oxidative stress; positive regulation of inflammatory response; positive regulation of cell migration
Research Articles on JAK2
1. HDAC2 might be a downstream effector of Jak2 to mediate cardiac hypertrophic response by pressure overload or Ang-II.
Precautions
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Disclaimer
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