SSC1; Aquaglyceroporin-9; Small solute channel 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Aquaporin 9 (AQP9).
No significant cross-reactivity or interference between Aquaporin 9 (AQP9) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AQP9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2020686 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 9 (AQP9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP9. The ELISA analytical biochemical technique of the MBS2020686 kit is based on AQP9 antibody-AQP9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 9 (AQP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 9 (AQP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 9 (AQP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aquaporin 9 (AQP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Metabolic pathway; Infection immunity; Developmental science

31,431 Da

aquaporin 9

aquaporin-9

Aquaporin-9

SSC1; AQP-9??[Similar Products]

AQP9_HUMAN

The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Channel, misc.; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; basolateral plasma membrane; plasma membrane; integral to membrane

Molecular Function: purine transmembrane transporter activity; water transporter activity; pyrimidine transmembrane transporter activity; amine transmembrane transporter activity; urea transmembrane transporter activity; carboxylic acid transmembrane transporter activity; polyol transmembrane transporter activity; water channel activity; porin activity; glycerol channel activity

Biological Process: polyol transport; glycerol transport; cellular water homeostasis; metabolic process; amine transport; water transport; carboxylic acid transport; response to osmotic stress; excretion; purine transport; response to mercury ion; response to organic substance; water homeostasis; pyrimidine transport; transport; canalicular bile acid transport; immune response; transmembrane transport

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