AQP9 (Aquaporin-9)/Aquaglyceroporin-9/Small solute channel 1

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of AQP9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760373 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin-9 (AQP9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP9. The ELISA analytical biochemical technique of the MBS760373 kit is based on AQP9 antibody-AQP9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human AQP9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human AQP9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AQP9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman AQP9 can be calculated.

31,431 Da

aquaporin 9

aquaporin-9

Aquaporin-9

SSC1; AQP-9??[Similar Products]

AQP9_HUMAN

The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, multi-pass; Transporter; Channel, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q

Cellular Component: basolateral plasma membrane; integral to membrane; integral to plasma membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: amine transmembrane transporter activity; carboxylic acid transmembrane transporter activity; glycerol channel activity; polyol transmembrane transporter activity; porin activity; purine transmembrane transporter activity; pyrimidine transmembrane transporter activity; urea transmembrane transporter activity; water channel activity; water transporter activity

Biological Process: amine transport; canalicular bile acid transport; carboxylic acid transport; excretion; glycerol transport; immune response; metabolic process; polyol transport; purine transport; pyrimidine transport; response to mercury ion; response to organic substance; response to osmotic stress; transmembrane transport; transport; water homeostasis; water transport

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