Product Name
NR0B1, siRNA
Popular Item
Full Product Name
NR0B1 siRNA (Mouse)
Product Synonym Names
AHCH; DAX1; Nuclear receptor subfamily 0 group B member 1; Nuclear receptor DAX-1
Product Gene Name
NR0B1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61066
Specificity
NR0B1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NR0B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NR0B1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NR0B1 sirna
siRNA to inhibit NR0B1 expression using RNA interference
Applications Tested/Suitable for NR0B1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NR0B1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031456.1
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NCBI GenBank Nucleotide #
NM_007430.5
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UniProt Primary Accession #
Q61066
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UniProt Secondary Accession #
O09068; O09147[Other Products]
UniProt Related Accession #
Q61066[Other Products]
NCBI Official Full Name
nuclear receptor subfamily 0 group B member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 0, group B, member 1
NCBI Official Symbol
Nr0b1??[Similar Products]
NCBI Official Synonym Symbols
AHX; Ahc; Ahch; Dax1; DAX-1
??[Similar Products]
NCBI Protein Information
nuclear receptor subfamily 0 group B member 1
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 1
UniProt Synonym Protein Names
Nuclear receptor DAX-1
Protein Family
Nuclear receptor subfamily
UniProt Gene Name
Nr0b1??[Similar Products]
UniProt Synonym Gene Names
Ahch; Dax1??[Similar Products]
UniProt Entry Name
NR0B1_MOUSE
NCBI Summary for NR0B1
This gene encodes an orphan nuclear receptor protein that plays a key role in differentiation of the gonads. This protein regulates steroidogenic factor 1 (Sf-1) in a dose-dependent manner, sometimes functioning as a repressor of SF-1 target genes, and sometimes functioning as a co-activator. Overexpression of this gene can cause feminization of the XY male gonads. This gene is also involved in the maintenance of embryonic stem cell pluripotancy. Mutations in the related gene in human cause congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, May 2015]
UniProt Comments for NR0B1
NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.
Protein type: Nuclear receptor
Cellular Component: polysomal ribosome; membrane; cytoplasm; nucleus
Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; RNA binding; transcription factor binding; steroid hormone receptor binding; DNA hairpin binding; protein binding; DNA binding; AF-2 domain binding; sequence-specific DNA binding; double-stranded DNA binding; steroid hormone receptor activity; transcription factor activity; transcription corepressor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; gonad development; transcription, DNA-dependent; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; sex determination; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; regulation of transcription, DNA-dependent; male sex determination; spermatogenesis; steroid hormone mediated signaling; cell differentiation; negative regulation of transcription, DNA-dependent; steroid biosynthetic process
Research Articles on NR0B1
1. Here we review the current knowledge on properties, functions and mechanisms of DAX1 action.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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