For Research Use Only. Not for use in diagnostic procedures.

This assay recognizes recombinant and natural rat AQP-9. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Aqp9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS288197 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin-9 (Aqp9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Aqp9. The ELISA analytical biochemical technique of the MBS288197 kit is based on Aqp9 antibody-Aqp9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Aqp9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Aqp9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AQP-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

31,871 Da

aquaporin 9

aquaporin-9

Aquaporin-9

AQP-9??[Similar Products]

AQP9_RAT

solute channel involved in passage of carbamides, polyols, purines, and pyrimidines [RGD, Feb 2006]

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Channel, misc.; Transporter, aquaporin family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: basolateral plasma membrane; integral to membrane; intracellular membrane-bound organelle

Molecular Function: glycerol channel activity; purine transmembrane transporter activity; pyrimidine transmembrane transporter activity; urea transmembrane transporter activity; water channel activity

Biological Process: amine transport; canalicular bile acid transport; glycerol transport; polyol transport; purine transport; pyrimidine transport; response to mercury ion; response to organic substance; water transport

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