Product Name
alpha Sarcoglycan (SGCA), Monoclonal Antibody
Full Product Name
Anti-alpha Sarcoglycan Rabbit Monoclonal Antibody
Product Synonym Names
Alpha-sarcoglycan; Alpha-SG; 50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2; SGCA; ADL; DAG2
Product Gene Name
anti-SGCA antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q16586
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human alpha Sarcoglycan
Subcellular Localization
Cell membrane, sarcolemma; Single-pass type I membrane protein. Cytoplasm, cytoskeleton.
Tissue Specificity
Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-SGCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SGCA antibody
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Product Categories/Family for anti-SGCA antibody
Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Proteins; glucosaminoglycans
Applications Tested/Suitable for anti-SGCA antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SGCA antibody
WB: 1:500-1:2000
IHC: 1:100-1:500
IP: 1:40
Western Blot (WB) of anti-SGCA antibody
Western blot analysis of alpha Sarcoglycan expression in Human skeletal muscle lysate (MBS1750028).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SGCA
NCBI/Uniprot data below describe general gene information for SGCA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000014.1
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NCBI GenBank Nucleotide #
NM_000023.3
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UniProt Primary Accession #
Q16586
[Other Products]
UniProt Secondary Accession #
Q13710; Q13712; A6NEB8; A8K3K7[Other Products]
UniProt Related Accession #
Q16586[Other Products]
Molecular Weight
42875 MW
NCBI Official Full Name
alpha-sarcoglycan isoform 1
NCBI Official Synonym Full Names
sarcoglycan alpha
NCBI Official Symbol
SGCA??[Similar Products]
NCBI Official Synonym Symbols
ADL; DAG2; 50DAG; DMDA2; LGMD2D; SCARMD1; adhalin
??[Similar Products]
NCBI Protein Information
alpha-sarcoglycan
UniProt Protein Name
Alpha-sarcoglycan
UniProt Synonym Protein Names
50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2
Protein Family
Alpha-sarcoglycan
UniProt Gene Name
SGCA??[Similar Products]
UniProt Synonym Gene Names
ADL; DAG2; Alpha-SG; 50DAG??[Similar Products]
NCBI Summary for SGCA
This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Comments for SGCA
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Research Articles on SGCA
1. Pathogenic mutations were found in SGCA from Egyptian families with limb-girdle muscular dystrophy.
Precautions
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Disclaimer
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