Product Name
C/EBP epsilon (CEBPE), Polyclonal Antibody
Full Product Name
Anti-C/EBP epsilon (pT74) Antibody
Product Synonym Names
CCAAT/enhancer-binding protein epsilon; C/EBP epsilon
Product Gene Name
anti-CEBPE antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15744
Species Reactivity
Human, Mouse, Rat, Sheep
Specificity
Recognizes endogenous levels of C/EBP epsilon (pT74) protein.
Purity/Purification
Purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human C/EBP epsilon.
Preparation and Storage
Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-CEBPE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CEBPE antibody
Rabbit polyclonal antibody to C/EBP epsilon (pT74)
Applications Tested/Suitable for anti-CEBPE antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CEBPE antibody
WB: 1:500-1:1000
IHC: 1:50-1:100
IF/ICC: 1:100-1:500
Western Blot (WB) of anti-CEBPE antibody
Western blot analysis of C/EBP epsilon (pT74) expression in Hela (A), mouse liver (B), Raw264.7 (C), rat liver (D) whole cell lysates.
Immunohistochemistry (IHC) of anti-CEBPE antibody
Immunohistochemical analysis of C/EBP epsilon (pT74) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescence (IF) of anti-CEBPE antibody
Immunofluorescent analysis of C/EBP epsilon (pT74) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NCBI/Uniprot data below describe general gene information for CEBPE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001796.2
[Other Products]
NCBI GenBank Nucleotide #
NM_001805.3
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UniProt Primary Accession #
Q15744
[Other Products]
UniProt Secondary Accession #
Q15745; Q8IYI2; Q99803[Other Products]
UniProt Related Accession #
Q15744[Other Products]
Molecular Weight
30,603 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein epsilon
NCBI Official Synonym Full Names
CCAAT enhancer binding protein epsilon
NCBI Official Symbol
CEBPE??[Similar Products]
NCBI Official Synonym Symbols
CRP1; C/EBP-epsilon
??[Similar Products]
NCBI Protein Information
CCAAT/enhancer-binding protein epsilon
UniProt Protein Name
CCAAT/enhancer-binding protein epsilon
Protein Family
CCAAT/enhancer-binding protein
UniProt Gene Name
CEBPE??[Similar Products]
UniProt Synonym Gene Names
C/EBP epsilon??[Similar Products]
NCBI Summary for CEBPE
The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for CEBPE
Transcriptional activator (PubMed:26019275). C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation (PubMed:10359588).
Research Articles on CEBPE
1. CEBPE expression was highest in multipotent progenitor cells (S1) and declined sharply as cells progressed to B-cell-committed progenitors, including pre-B-I cells (S2), pre-B-II cells (S3) and immature B cells (S4)
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