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IDH2, Monoclonal Antibody

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產品名稱: IDH2, Monoclonal Antibody
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IDH2, Monoclonal Antibody


IDH2, Monoclonal Antibody  的詳細介紹
Product Name

IDH2, Monoclonal Antibody

Full Product Name

Anti-IDH2 Monoclonal Antibody

Product Gene Name

anti-IDH2 antibody

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P48735
Clonality
Monoclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
No cross reactivity with other proteins.
Purity/Purification
Affinity-Chromatography
Form/Format
Liquid, in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human IDH2 Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Preparation and Storage
Store at 4 degree C short term. Store at -20 degree C long term
Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-IDH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
anti-IDH2 antibody
Rabbit IgG Monoclonal antibody for P48735 detection. Tested positive for WB IHC FC in Human Mouse Rat
Applications Tested/Suitable for anti-IDH2 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-IDH2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
FC/FACS: 1:100

Western Blot (WB) of anti-IDH2 antibody
Figure 1. Western blot analysis of IDH2 using anti-IDH2 antibody (M00510).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDH2.
anti-IDH2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for IDH2. It may not necessarily be applicable to this product.
NCBI GI #
583966148
NCBI GeneID
3418
NCBI Accession #
NP_001276839.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001289910.1 [Other Products]
UniProt Primary Accession #
P48735 [Other Products]
UniProt Secondary Accession #
Q96GT3; B2R6L6; B4DFL2[Other Products]
UniProt Related Accession #
P48735[Other Products]
Molecular Weight
45,180 Da
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NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase (NADP(+)) 2, mitochondrial
NCBI Official Symbol
IDH2??[Similar Products]
NCBI Official Synonym Symbols
IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
??[Similar Products]
NCBI Protein Information
isocitrate dehydrogenase [NADP], mitochondrial
UniProt Protein Name
Isocitrate dehydrogenase [NADP], mitochondrial
UniProt Synonym Protein Names
ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Protein Family
Isocitrate dehydrogenase
UniProt Gene Name
IDH2??[Similar Products]
UniProt Synonym Gene Names
IDH??[Similar Products]
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NCBI Summary for IDH2
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for IDH2
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Research Articles on IDH2
1. In this review, we summarize current knowledge regarding the function of normal and mutated IDH(socitrate dehydrogenases 1 and 2 ), explain the possible mechanisms through which these mutations might drive malignant transformation of progenitor cells in the central nervous system, and provide a comprehensive review of potential treatment strategies for IDH-mutated malignancies, focusing on gliomas.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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