Anti-NR0B1 / DAX1 Antibody (N-Terminus) IHC-plus; NR0B1; AHX; AHC; AHCH; DAX1; DSS; DAX 1; DAX-1; Adrenal hypoplasia congenita; Dosage-sensitive sex reversal; GTD; HHG; Nuclear hormone receptor; NROB1; Nuclear receptor DAX-1; SRXY2; Nuclear receptor DAX1; Dax-1 receptor; Human NR0B1; DAX1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Human DAX1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Immunoaffinity Purified

PBS, 0.1% sodium azide.

0.7 mg/ml (lot specific)

Long term: -70 degree C; Short term: +4 degree C

Small volumes of anti-NR0B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DAX-1 (DSS-AHC critical region on the X chromosome, gene 1) is an orphan NRO Knirps-Like nuclear receptor that is involved in adrenal gland and gonad development. DAX-1 has been shown to negatively regulate transcription of the nuclear receptor steroidogenic factor 1 (SF-1). DAX-1 is also involved in the development and differentiation of anterior pituitary cells and in steroidogenesis.

Subfamily:-NR0-Knirps-like">Family: NHR
Subfamily: NR0 Knirps-like

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

ELISA, IHC-P (10 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Anti-DAX1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

43,590 Da

nuclear receptor subfamily 0, group B, member 1

nuclear receptor subfamily 0 group B member 1; nuclear receptor DAX-1; nuclear hormone receptor; DSS-AHC critical region on the X chromosome protein 1

Nuclear receptor subfamily 0 group B member 1

AHC; DAX1??[Similar Products]

NR0B1_HUMAN

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: Xp21.3

Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; nucleus

Molecular Function: DNA hairpin binding; protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; AF-2 domain binding; sequence-specific DNA binding; RNA binding; steroid hormone receptor activity; transcription corepressor activity; transcription factor binding; steroid hormone receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; hypothalamus development; negative regulation of steroid hormone receptor signaling pathway; gonad development; intracellular receptor-mediated signaling pathway; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; pituitary gland development; male sex determination; gene expression; steroid hormone mediated signaling; spermatogenesis; negative regulation of transcription, DNA-dependent; steroid biosynthetic process

Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.